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. 2019 Dec 29;16(9):1697–1710. doi: 10.1080/15548627.2019.1704118

Figure 7.

Figure 7.

Effects of different coiled-coil domains of VP3 on replication of virus. (A) CC3 domain is critical for VP3 promoting replication of Avibirnavirus. WT or PIK3C3 KO DF-1 cell lines stably expressing VP3, VP3∆CC1, VP3∆CC2, VP3∆CC3 or not were infected Avibirnavirus at MOI = 0.01 for 48 h. The virus was collected for titer detection. (B) CC3 domain is critical for VP3 inhibiting replication of rAdV. The rAdV expressing VP3, VP3∆CC1, VP3∆CC2, VP3∆CC3 or not were rescued from 293T cells. WT or PIK3C3 KO 293T cell lines were infected with these rescued rAdVs. Titers of progeny virus were then tested in 293T cells. Error bars: Mean ± SD of 3 independent tests. One-way ANOVA; *p < 0.05; ** p < 0.01; ***p < 0.001 compared to control.