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. 2019 Nov 7;16(10):1786–1806. doi: 10.1080/15548627.2019.1687212

Figure 3.

Figure 3.

STYK1 colocalizes to autophagosomes and stimulates their formation. (A-F) Colocalization of STYK1-mCherry and ATG14-GFP (A), ZFYVE1-GFP (C), and WIPI1-GFP (E) in HeLa cells. The number of ATG14-GFP (B), ZFYVE1-GFP (D), and WIPI1-GFP (F) puncta before or after STYK1 overexpression was quantified using ImageJ software (n = 10, respectively). Scale bars: 10 µm. (G) The colocalization of ATG14-GFP, ZFYVE1-GFP, WIPI1-GFP and STYK1-mCherry was quantified. Scale bars: 10 µm. (H) Immunofluorescence analysis of ATG14-GFP puncta after co-transfection with ATG14-GFP, STYK1-mCherry and ULK1 siRNA into the HeLa cells for 48 h. Scramble siRNA was used as a negative control. The number of ATG14 puncta was quantified (n = 10). Scale bars: 10 µm. *P < 0.05; **P < 0.01; ***P < 0.001. Data are presented as mean ± SD.