Fig. 3. Site-directed mutation of position 6.55 and its impact on the coupling outcome at the D2R receptor. The coupling outcome of the D2R WT (blue line) compared to the H6.55N (black line) and H6.55F (orange line) mutants are plotted upon receptor stimulation with dopamine (A and D), (S)-5-OH-DPAT (B and E) and (R)-7-OH-DPAT (C and F). Concentration–response curves were obtained from 3 independent experiments. Baseline uBRET values were subtracted from concentration–response curves. For corresponding pEC50 and Emax values see Table S3C to H.†.
