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. 2020 Jul 9;17(8):1828–1840. doi: 10.1080/15548627.2020.1783833

Figure 1.

Figure 1.

DNA damage and oxidative stress induce eMI in the larval fat body. (A, B) Compared to untreated control (A), treatment of larvae with the DNA damaging agent Eto for 25 h robustly induces eMI puncta. (C, D) Oxidative stress induced by paraquat induces eMI. (C) Untreated control, (D) fat body of paraquat-treated larvae. eMI sensor is shown in red, (endo)lysosomes in green, nuclei are in blue. A’-D’ and A” to D”: single-channel image of indicated channels. Scale bar: 20 µm. (E) Quantification of eMI puncta per cell at indicated time points. Grey: untreated, blue: paraquat-, and magenta: Eto-treated. (F) Quantification of eMI puncta upon treatment with 50 µM doxorubicin (magenta). (G) Quantification of eMI puncta upon treatment with indicated concentrations of menadione bisulfite (blue). E: One-way ANOVA (Dunnett corrections) p < 0.05; F: Unpaired T-test ****, P < 0.0001. G: One-way ANOVA (Tukey corrections) p < 0.0001. *, P < 0.05; **, P < 0.01, ****, P < 0.0001. Unpaired T-test results are indicated as “t-values”