Table 1. Detection of SARS-CoV-2 by different techniques in samples collected from 19 farmed mink, Poland*.
Sample name | Cp for RdRP gene† | Cp for E gene† | Antigen in swab specimen | Antigen in serum, concentration of N protein, pg/mL | Sequence obtained |
---|---|---|---|---|---|
mink_4 | ND | 24.90 | Strong positive | 46.86 | Full |
mink_5 | 24.36 | 16.88 | Strong positive | 3.30 | Full |
mink_6 | ND | 34.88 | Negative | ND | Partial |
mink_20 | ND | ND | Positive | 132.24 | Partial |
mink_27 | ND | 35.06 | Negative | ND | Partial |
mink_36 | ND | ND | Positive | 271.30 | Partial |
mink_39 | ND | ND | Negative | 35.59 | Partial |
mink_42 | ND | 25.62 | Positive | ND | Full |
mink_46 | 34.93 | 22.75 | Positive | ND | Full |
mink_48 | 29.89 | 22.52 | Strong positive | ND | Full |
mink_49 | ND | 27.79 | Positive | ND | Full |
mink_50 | 32.39 | 21.61 | Strong positive | ND | Full |
mink_63 | ‒ | ‒ | Negative | 19.30 | Partial |
mink_67 | 36.88 | 23.34 | Positive | ND | Full |
mink_70 | ND | 24.40 | Positive | ND | Partial |
mink_76 | 32.53 | 22.73 | Positive | ND | Full |
mink_77 | 32.87 | 20.30 | Strong positive | ND | Full |
mink_83 | 15.39 | 21.77 | Strong positive | 91.57 | Full |
mink_88 | ND | 33.99 | Positive | ND | Full |
NTC | ND | ND | ND | ND | ND |
Positive control | 16.91 | 23.31 | ND | ND | ND |
*Samples in bold are considered positive for SARS-CoV-2 by nucleic acid amplification, serologic analysis, or next-generation sequencing. Cp, crossing point; E, envelope; N, nucleocapsid; ND, not detected; NTC, no template control; RdRp, RNA-dependent RNA polymerase; SARS-CoV-2, severe acute respiratory coronavirus 2. †By reverse transcription quantitative PCR.