FIG. 2.
Presence of Ada1 in the SAGA complex. HAT complexes were derived from wild-type (PSY316) and ada1Δ (SB11) yeast strains through the Mono Q column step of the previously described purification procedure. (A) Fluorographs of nucleosome acetylation assays of the even-numbered column fractions. The arrows denote the relative positions of the four histone proteins as separated on SDS–18% polyacrylamide gels; visualized species contain acetyl groups labeled with 3H. Wild-type fractions displaying the H3/H2B-acetylating activities of the Ada and SAGA complexes are indicated at the top. (B) Western blots of the fractions, visualized with dilutions of antisera raised against Ada1, Ada2, or Gcn5 protein.