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. 2021 Aug 13;17(8):e1009753. doi: 10.1371/journal.pgen.1009753

Fig 1. The dynamic localization of MTL5 in spermatocytes of meiotic prophase I.

Fig 1

(A) Immunostaining of MTL5 (red) and γH2AX (green) in testicular sections of 10-week-old wild-type mice. Nuclei were counterstained with Hoechst 33342 (blue). Enlarged views (bottom) are indicated by white boxes (top). White arrowheads indicate pachytene or diplotene spermatocytes. Blue arrowheads indicate leptotene or zygotene spermatocytes. Scale bars, 50 μm. (B) Immunostaining for MTL5 (red) and γH2AX (green) in smeared testicular cells of 10-week-old wild-type mice for MTL5 (red) and γH2AX (green). Nuclei were counterstained with Hoechst 33342 (blue). White arrowheads indicate pachytene or diplotene spermatocytes. Green dotted ovals indicate zygotene spermatocytes. Scale bars, 10 μm. (C) Immunostaining of MTL5 (green) and PNA (red) in testicular sections of 10-week-old wild-type mice. Nuclei were counterstained with Hoechst 33342 (blue). Enlarged views are indicated by white boxes. Scale bars, 50 μm. (D) Immunostaining for SYCP3 (red), H1t (gray), and MTL5 (green) in spread spermatocytes of 10-week-old wild-type mice. Scale bars, 10 μm. (E) Western blot analyses were performed using cytoplasmic and nuclear protein extracts from 8, 10, 12, 15, 20, and 25 days post-partum (dpp) mouse testes. GAPDH and SYCP3 served as cytoplasmic and nuclear controls, respectively.