Fig 6. MTL5 C-terminal deletion leads to spermatogenesis arrest at pachytene stage in Mtl5^c-mu/c-mu mice.

(A) Testis morphology, (B) Ratios of testis/body weight and (C) Epididymal sperm counts of 10-week-old Mtl5^+/+, Mtl5^-/-, and Mtl5^c-mu/c-mu mice. (D) H&E staining of testicular and epididymal sections from 10-week-old mice. Scale bars, 50 μm. (E) Immunostaining for SYCP3 (green) and SYCP1 (red) in surface-spread spermatocytes from 10-week-old Mtl5^+/+, Mtl5^-/-, and Mtl5^c-mu/c-mu mice. Scale bars, 10 μm. (F) Percentages of spermatocytes at sub-stages of meiotic prophase I. The numbers in the brackets indicate the number of spermatocytes counted. Data are presented as mean ± SEM from at least four mice. P values were analyzed by One-way ANOVA. *p<0.05;****p<0.0001;NS, p>0.05.