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. 2021 Aug 25;10:e65872. doi: 10.7554/eLife.65872

Figure 8. MLL-HBO1 complex association is mediated by multiple contacts and in a chromatin-bound context containing H3K4 methylation.

Figure 8.

(A) Association of the MLL bait protein with the HBO1 complex. Immunoprecipitation (IP)-western blotting of the chromatin fraction of HEK293T cells transiently expressing FLAG-tagged MLL 869/1124 construct and Xpress-tagged HBO1 complex components was performed. (B) Influence of each HBO1 complex component for MLL-HBO1 complex association. (C) Domain mapping of ING5 responsible for MLL-HBO1 complex association. (D) Models of MLL-ELL- and nucleoporin-98 (NUP98)-HBO1-mediated gene activation.