| Reagent or Resource | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Fasciclin III (Fas3) (1:50) | DSHB | Cat# 7G10 anti-Fasciclin III; RRID: AB_528238 |
| Rabbit polyclonal anti-GFP (1:500) | Invitrogen | Cat# A-6455; RRID: AB_221570 |
| Goat polyclonal anti-Vasa (1:200) | Santa Cruz | Cat# sc26877; RRID: AB_793877 |
| Chicken polyclonal anti-GFP (1:500) | Abcam | Cat# ab13970; RRID: AB_300798 |
| Rat monoclonal anti-N Cadherin (1:20) | DSHB | Cat# DN-ex #8; RRID: AB_528121 |
| Rat monoclonal anti DE-Cadherin (1:20) | DSHB | Cat# DCad2; RRID: AB_528120 |
| Mouse monoclonal anti-Eyes absent (Eya) (1:20) | DSHB | Cat# eya10H6; RRID: AB_528232 |
| Rat monoclonal anti-Vasa | DSHB | Cat# anti-vasa; RRID: AB_760351 |
| Mouse monoclonal anti- β-galactosidase (1;500) | Promega | Cat# Z3781; RRID: AB_430877 |
| Mouse monoclonal anti-Dp (1:5) | N. Dyson (MGH Charlestown, USA | N/A |
| Rabbit polyclonal anti-Zfh1 (1:1000) | K. White (University of Chicago, USA) | N/A |
| Guinea pig polyclonal anti-Traffic jam (1:3000) | D. Godt (University of Toronto, Canada) | N/A |
| Cy3-AffiniPure Donkey Anti-Mouse IgG (1:400) | Jackson ImmunoResearch Labs | Cat# 715-165-150; RRID: AB_2340813 |
| Alexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 711-545-152; RRID: AB_2313584 |
| Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L)(1:400) | Jackson ImmunoResearch Labs | Cat# 711-165-152; RRID: AB_2307443 |
| Cy5-AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 711-175-152; RRID: AB_2340607 |
| Alexa Fluor 488-AffiniPure Donkey Anti-Rat IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 712-545-150; RRID: AB_2340683 |
| Cy3-AffiniPure Donkey Anti-Rat IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 712-165-150; RRID: AB_2340666 |
| Cy5-AffiniPure Donkey Anti-Rat IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 712-175-150; RRID: AB_2340671 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 703-545-155; RRID: AB_2340375 |
| Cy3-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 703-165-155; RRID: AB_2340363 |
| Cy5-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 703-175-155; RRID: AB_2340365 |
| Cy3-AffiniPure Donkey Anti-Guinea Pig IgG (1:400) | Jackson ImmunoResearch Labs | Cat# 706-165-148; RRID: AB_2340460 |
| Cy5-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 706-175-148; RRID: AB_2340462 |
| Cy3-AffiniPure Donkey Anti-Goat IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 705-165-003; RRID: AB_2340411 |
| Alexa Fluor 647 AffiniPure Donkey Anti-Goat IgG (H+L) (1:400) | Jackson ImmunoResearch Labs | Cat# 705-605-003; RRID: AB_2340436 |
| Chemicals, peptides, and recombinant proteins | ||
| VECTASHIELD Mounting Medium | Vector Laboratories | Cat# H-1000; RRID: AB_2336789 |
| VECTASHIELD Mounting Medium with DAPI | Vector Laboratories | Cat# H-1200; RRID: AB_2336790 |
| Paraformaldehyde, 16% w/v aq. soln., methanol free | Thermo Fisher Scientific | Cat# 43368-9L |
| TO-PRO-3 iodide (1 μM) | Invitrogen | Cat# T3605 |
| 5-ethynyl-2’-deoxyuridine (EdU) | Invitrogen | Cat# A10044 |
| AF405 picolylazide | Click chemistry tools | Cat# 1308-5 |
| Tris(3-Hydroxyproyltriazolylmethyl)Amine | Sigma Aldrich | Cat# 762342 |
| Sodium Ascorbate | Sigma Aldrich | Cat# PHR1279 |
| Copper(II) sulfate | Fisher Scientific | Cat# 15617730 |
| Molasses | Labscientific | Cat# FLY-8008-16 |
| Agar | Mooragar | Cat# 41004 |
| Cornmeal | LabScientific | Cat# FLY-8010-20 |
| Yeast | LabScientific | Cat#FLY-8040-20F |
| Tegosept | Sigma | Cat# H3647-1KG |
| Reagent alcohol | Fisher | Cat# A962P4 |
| Propionic acid | Fisher | Cat# A258500 |
| Experimental models: organisms/strains | ||
| D. melanogaster; OregonR | Bach lab | N/A |
| D. melanogaster; P[GawB]NP1624/CyO (tj-GAL4) | Bach lab | Kyoto Stock Center: 104055 Flybase: FBst0302922 |
| D. melanogaster; nos-GAL4-VP16 | Ruth Lehmann (Whitehead Institute, USA) | N/A |
| D. melanogaster; C587-GAL4 | Ruth Lehmann (Whitehead Institute, USA) | N/A |
| D. melanogaster; fng-GAL4 | Stephen Dinardo (Perelman School of Medicine, University of Pennsylvania, USA) | N/A |
| D. melanogaster; eyaA3-GAL4 (denoted eya-GAL4) | Stephen Dinardo (Perelman School of Medicine, University of Pennsylvania, USA) | N/A |
| D. melanogaster; upd-GAL4 | Bach lab | N/A |
| D. melanogaster; tub-GAL80TS | Bloomington Drosophila stock Center (BDSC) | BDSC_7017 FlyBase: FBst0007017 |
| D. melanogaster; hh-LacZ | Bach lab | BDSC_5530 FlyBase: FBst0005530 |
| D. melanogaster; upd-LacZ | Bach lab | N/A |
| D. melanogaster; hh-GAL80 | This paper | N/A |
| D. melanogaster; hh-QF | This paper | N/A |
| D. melanogaster; w1118; P[w[+mC]=UAS-lacZ.NZ]J312. Insertion on III | BDSC | BDSC_3956 FlyBase: FBst003956 |
| D. melanogaster; w1118; P[w[+mC]=UAS-lacZ.NZ]20b.Insertion on II | BDSC | BDSC_3955 FlyBase: FBst003955 |
| D. melanogaster; UAS-GFP; Ubi-p63E(FRT.STOP)Stinger | BDSC | BDSC_32251 |
| D. melanogaster; w1118; P[GD4444]v12722 (Dp-RNAi) | Vienna Drosophila Resource Center (VDRC) | VDRC_v12722 FlyBase: FBst0450633 |
| D. melanogaster; FRT42D, Dpa3/CyO | M. Frolov, University of Illinois at Chicago, USA | Flybase: FBgn0011763 |
| D. melanogaster; FRT42D, Dpa4/CyO | M. Frolov, University of Illinois at Chicago, USA | Flybase: FBgn0011763 |
| D. melanogaster; UAS-Dp | M. Frolov, University of Illinois at Chicago, USA | Flybase: FBgn0011763 |
| D. melanogaster; FRT82BE2f1729 | M. Frolov, University of Illinois at Chicago, USA | FlyBase: FBgn0011766 |
| D. melanogaster; y1, v1; P[TRiP.JF02519]attP2/TM3, Sb1(Dp-RNAi) | BDSC | BDSC_30515 FlyBase: FBst0030515 |
| D. melanogaster, w1118; P[GD4448]v15886 (E2f1-RNAi) | VDRC | VDRC: v15886 FlyBase: FBst0452055 |
|
D. melanogaster; UAS-Rbf280 Insertion on III |
W. Deng (Tulane University, USA) | BDSC_50748 Flybase: FBst0050748 |
| D. melanogaster; act>y[+]>LHV2-86Fb,13XlexAop2-myr::GFP (FLEX-AMP) | C. Desplan (New York University, USA) | N/A |
| D. melanogaster; UAS-FLP | BDSC | BDSC_4539 |
| D. melanogaster; QUAS-FLP | BDSC | BDSC_30126 |
| D. melanogaster; w1118; P[GD15843]v46260 (Fs-RNAi) | VDRC | VDRC v46260 FlyBase: FBst0466595 |
| D. melanogaster; y1v1;P[TRiP.HMJ03135]attP40 (daw-RNAi) | BDSC | BDSC_50911 Flybase: FBst0050911 |
|
D. melanogaster; P[KK110248]VIE-260B (daw-RNAi) |
VDRC | VDRC #v105309 Flybase: FBst0477137 |
| D. melanogaster; y1sc∗v1sev21;P[TRiP.HMS01110]attP2 (daw-RNAi) | BDSC | BDSC_34974 Flybase: FBst0034974 |
| D. melanogaster; y1v1; P[TRiP.GL01476]attP2 (smox-RNAi) | BDSC | BDSC_43138 Flybase: FBst0043138 |
| D. melanogaster; y1w∗; Mi[MIC]FsMI01433 (FsMI01433) | BDSC | BDSC_33121 Flybase: FBst0033121 |
| D. melanogaster; y1w∗; Mi[MIC]FsMI11350(FsMI11350) | BDSC | BDSC_56310 Flybase: FBst0056310 |
| D. melanogaster; y1w∗;FsMI04308-TG4.1CG8079MI04308-TG4.1-X (FsTJ4.1) | BDSC | BDSC_66838 (lost during the pandemic) |
|
D. melanogaster; y1w∗; Mi[PT-GFSTF.1]FsMI04308-GFSTF.1CG8079MI04308-GFSTF.1-X /CyO (FsGFSTF.1 or Fs-GFP) |
BDSC | BDSC_65327 Flybase: FBst0065327 |
| D. melanogaster; Fsnull | This paper | N/A |
| D. melanogaster; UAS-Fs | O’Connor lab | N/A |
| D. melanogaster; Fs-GAL4 (FsMI-GAL4) | This paper | N/A |
| D. melanogaster; UAS-esgNLAP | L. Jones (UCSF, USA) | N/A |
| D. melanogaster; UAS-NLAP | L. Jones (UCSF, USA) | N/A |
| D. melanogaster; Actβ-GAL4 | O’Connor lab | N/A |
| D. melanogaster; myo-GAL4 | O’Connor lab | N/A |
| D. melanogaster; y1w∗; Mi[MIC]dawMI05383 (daw-GFP) | BDSC | BDSC_43001 Flybase: FBst0043001 |
| D. melanogaster; UAS-babo-RNAi. | O’Connor lab | N/A |
| D. melanogaster; UAS-baboQD | O’Connor lab | N/A |
|
D. melanogaster; PBac[fTRG00506. sfGFP-TVPTBF]VK00033 (ActβfTRG00506.sfGFP-TVPTBF) |
VDRC | VDRC #v318136 Flybase: FBst0491562 |
|
D. melanogaster; PBac[fTRG00161.sfGFP-TVPTBF]VK00033 (myofTRG00161.sfGFP-TVPTBF) |
VDRC | VDRC #v318065 Flybase: FBst0491390 |
|
D. melanogaster; PBac[fTRG00444.sfGFP-TVPTBF]VK00033 (babofTRG00444.sfGFP-TVPTBF) |
VDRC | VDRC #v318433 Flybase: FBst0491516 |
| D. melanogaster; w∗; P[GawB]dawNP4661/ CyO (dawNP4661-GAL4) | Kyoto Stock Center | Kyoto #113490 Flybase: FBst0316217 |
| D. melanogaster; y∗w∗; P[GawB]dawNP6274/ CyO (dawNP6274-GAL4) | Kyoto Stock Center | Kyoto #105179 Flybase: FBst0304038 |
| Oligonucleotides | ||
| gRNA Fs 1 | 5' CCGGTTGCATCATGTATCTTGGC 3' | IDTDNA |
| gRNA Fs 2 | 5' CCAACTGGAGGTCGCCTATCGGG 3' | IDTDNA |
| qPCR primer Fs-fwd: | 5’-AGTGTCATATATACTCTCCGCATGT-3’ | IDTDNA |
| qPCR primer Fs-rev: | 5’-ACAGCAACTGCTTTTTAACTATGCC-3’ | IDTDNA |
| qPCR primer α-tub84B-fwd: | 5’-TCGTTTTACGTTTGTCAAGCCTC-3’ | IDTDNA |
| qPCR primer α-tub84B-rev: | 5’-GAGATACATTCACGCATATTGAGTT-3’ | IDTDNA |
| qPCR primer daw-fwd | 5’-CCCATCTTCGACGGGATGAC-3’ | IDTDNA |
| qPCR primer daw-rev | 5’-TTGCACTCGACCTCCTCTCT-3’ | IDTDNA |
| Software and algorithms | ||
| ImageJ/Fiji | Fiji | http://fiji.sc/ |
| Photoshop/Illustrator | Adobe | https://www.adobe.com/products/ |
| Prism | GraphPad | https://www.graphpad.com |
| ZEN | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Excel | Microsoft | https://products.office.com/en-us/excel |
| Imaris | Oxford Instruments | https://imaris.oxinst.com/ |
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