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. 2021 Aug 25;95(18):e00353-21. doi: 10.1128/JVI.00353-21

TABLE 2.

Primers for amplification of enFeLV, FeLV-A, and FeLV-B env segments

FeLV type Orientation Primer sequence Primer location Amplicon size (kb)
FeLV-A Forward 5′-ACCCAAGCTAATGCCACCTC-3′ FeLV-A env ∼1.9
Reverse 5′-CCTCTAACTTCCTTGTATCTCATGG-3′ FeLV-A 3′-LTR
enFeLV Forward 5′-CTGACAGACGCCTTCCCTAC-3′ enFeLV env ∼2
Reverse 5′-CTAGGCTCATCTCTAGGGTCTATC-3′ enFeLV 3′LTR
FeLV-B Forward 5′-CAGATCAGGAACCATTCCCAGG-3′ enFeLV env and FeLV-B ∼1.8
Reverse 5-CCTCTATCTTCCTTGTATCTCATGG-3′ FeLV-A and FeLV-B 3′-LTR