Adsorption rate of phage PNJ1809-36 to host DE058 treated with different agents. (A) Adsorption rates of the host bacteria treated with proteinase K. Columns 1, 2, and 3 represent the respective added substances: 1, LB medium (negative control); 2, 20 mM Tris-HCl and 100 mM NaCl (pH 7.5); 3, 0.5 mg/ml proteinase K in 20 mM Tris-HCl and 100 mM NaCl (pH 7.5). (B) Adsorption rates of host bacteria treated with periodate. Columns 1, 2, 3, and 4 indicate the addition of different reagents: 1, LB medium (negative control); 2, 50 mM CH3COONa (pH 5.2); 3, 10 mM sodium periodate (NaIO4) and 50 mM sodium acetate (CH3COONa) (pH 5.2); 4, 100 mM NaIO4 and 50 mM CH3COONa (pH 5.2). **, P < 0.01.