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Effects of a panel of F412 mutations on the restriction function and steady-state expression level of SERINC5. (A and B) Restriction activity (A) and immunoblot analysis (B) of WT and variants of SERINC5-iHA having various amino acid substitutions at F412 against EnvNL pseudotyped HIV-1 NL43ΔEnvΔNef produced in HEK293T cells. Data are means and SD from 3 independent experiments.
