FIG 5.
Effects of the panel of F412 mutations of SERINC5 on the virus-cell fusion process. (A) HIV-1 NL43ΔEnvΔNef viruses pseudotyped without (mock) or with EnvNL supplemented with genes encoding BlaM-Vpr and SERINC5-iHA WT or a panel of F412 mutants were produced in HEK293T cells. Fusion formation between the resultant viral particles and the TZM-bl target cells, as assessed by fluorescence signals from the cleaved form of CCF4 (excitation of 447 nm), was analyzed by flow cytometry. The percentage of cells that successfully achieved virus-cell fusion is indicated in the dot plots. Data are representative of 3 independent experiments. (B) Correlation between the restriction function and fusion efficiency in the context of WT and the panel of F412 mutants of SERINC5. Fusion efficiency was normalized to the absence of SERINC5-iHA, which was set to 100%. Data are means from 3 independent experiments. The statistical significance was analyzed by using Pearson’s correlation coefficient test.