FIG 7.
Effects of F412A on SERINC5-mediated restriction of various retroviral envelopes. Infectivity values (left) of HIV-2 GL-ANΔEnvΔNef pseudotyped with HIV-2 EnvGH123 (A), SIVmacΔEnvΔNef pseudotyped with SIVmac239 Env (B), and HIV-1 NL43ΔEnvΔNef pseudotyped with MLV Envs (C) in the absence and presence of the indicated SERINC5. Fold difference in infectivity (as restriction activity; right) of the same pseudoviruses (A to C) between the absence and the presence of WT or F412A mutant of SERINC5-HA. The pseudoviruses were produced in the absence and the presence of WT or F412A mutant of SERINC5-HA in HEK293T cells and tested for the single-round infectivity as assessed in terms of firefly luciferase activity in MAGIC5 target cells; and the background levels were deducted. Data are means and SD from 3 independent experiments.