FIG 2.

Fc glycosylation does not affect the ability of PGT121 to recognize infected cells or its neutralization capacity. Cell surface staining of CEM.NKr CCR5⁺ cells infected with HIV-1_JRCSF (A), SHIV_AD8-EO (B), and SHIV_BG505 (C) was performed 48 h postinfection. Antibody binding was detected using Alexa Fluor 647-conjugated anti-human secondary Abs. Graphs represent the median fluorescence intensities (MFI) in the infected population (p24+ or p27+) determined from at least five independent experiments, with the error bars indicating means ± the standard errors of the mean (SEM). Statistical significance was tested using an unpaired t test or a Mann-Whitney U test based on statistical normality (****, P < 0.0001; ns, nonsignificant). (D to F) Lentiviral particles produced from HIV-1_JRCSF (D), SHIV_AD8-EO (E), and SHIV_BG505 (F) IMCs. Viruses were incubated with serial dilutions of trastuzumab and PGT121 MAbs at 37°C for 1 h prior to infection of TZM-bl target cells. The infectivity at each Ab concentration tested is shown as the percentage of infection without Ab for each virus. Quadruplicate samples were analyzed in each experiment. The data shown are the means of results obtained in at least three independent experiments. Error bars indicate means ± the SEM. Black histogram/curves represent 293F cell-derived MAbs and green histogram/curves represent plant-derived MAbs.