FIG 7.
Comparison of the overall structures of N. benthamiana expressed and mammalian expressed human Fc domains. Structural alignment of CH2-CH3 dimers (Fc), CH2-CH3 monomers, CH2 domains, and CH3 domains of N. benthamiana and mammalian expressed human Fcs including the following: a human fucosylated Fc lacking the terminal galactose (PDB ID 3AVE, yellow), an afucosylated Fc (2DTS, pink), and a fucosylated Fc containing a terminal galactose (5VGP, gray). N. benthamiana expressed Fcs are colored as in Fig. 6, with fucose colored green and galactose cyan. (B) Average RMSD values for main chain atom pairwise comparisons of CH2 domains, CH3 domains, CH2-CH3 monomers, and CH2-CH3 dimers (Fc domain) shown in tabular format color coded with smaller RMSD values green and larger RMSD values red.