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. 2021 Aug 25;95(18):e00878-21. doi: 10.1128/JVI.00878-21

FIG 6.

FIG 6

The G76A and C56U mutations increased the replication of the 3′-terminal H1-ssNCR-truncated mutant template in multitemplate RNP recombination assays. HEK-293T cells were cotransfected with pcDNA-PB2, pcDNA-PB1, pcDNA-PA-D108A, pcDNA-NP, pPOL1-HA, and pPOL1-mut-HA with G76A(A) or C56U(B). A polymerase containing an active site mutation (PB1a, D445A/446A) was used as negative control (NC). Accumulation of RNA 24 h posttransfection was analyzed by primer extension. The graph shows the mean intensity signals of mutant HA RNAs relative to those of wild-type HA RNAs. The data represent the means ± SEM of three independent experiments. Two-way analysis of variance (ANOVA) with Dunnett correction for multiple testing; asterisks represent a significant difference from wild-type virus as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.