FIG 3.

Amino acids 72 and 73 of E120R were essential for the E120R-mediated inhibitory effect on IFN-β production. (A to D) HEK-293T cells were transfected with 0.1 μg/well of IFN-β-Luc expression plasmid and 0.01 μg/well of pRL-TK plasmid along with 0.1 μg/well each of HA-cGAS and HA-STING expression plasmids and FLAG vector or FLAG-E120R or FLAG-E120R mutant expression plasmid. At 24 hpt, the promoter activation of IFN-β was determined by using the Dual-Luciferase assay kit. The expression of various E120R mutants was determined by Western blotting. C, cGAS; S, STING. Three independent experiments were performed with two technical replicates. Error bars show standard deviations. *, P < 0.05; **, P < 0.01.