FIG 5.

ASFV E120R-Δ72-73aa infection induced higher expression levels of IFN-β and ISGs in PAMs than infection by ASFV WT. (A and B) PAMs were infected with ASFV WT or ASFV E120R-Δ72-73aa (MOI of 1) for 0, 6, 12, or 24 h. (A) The cells were collected for detection of IFN-β and ISG15 mRNA expression. (B) The supernatants were collected at 0, 12, or 24 hpi, and the secreted IFN-β protein was measured using an ELISA kit. Two independent experiments were performed with similar results. Error bars show standard deviations. **, P < 0.01. (C) PAMs were mock infected or infected by equal amounts of ASFV WT or ASFV E120R-Δ72-73aa for 12 h. Transcriptome analysis was then carried out. Gene Ontology (GO) enrichment analysis of DEGs in PAMs infected by ASFV WT or ASFV E120R-Δ72-73aa was performed, and the top 20 most significant enrichment terms were extracted (P < 0.05). Three technical replicates were performed. (D) The heat map shows the top 100 significant DEGs in PAMs in response to ASFV WT or ASFV E120R-Δ72-73aa infection. Three technical replicates were performed. (E) Alignment of the E120R protein sequences of different ASFV strains reported in recent years.