FIG 6.

IRF3 was the potential target of ASFV E120R protein for suppression of IFN-β production. HEK-293T cells were transfected with 0.1 μg/well of IFN-β-Luc expression plasmid and 0.01 μg/well of pRL-TK plasmid along with 0.1 μg/well of FLAG vector or FLAG-E120R or FLAG-E120R dm expression plasmid and HA-tagged-cGAS, -STING, -TBK1, or -IRF3 expression plasmid. At 24 hpt, the activation of the IFN-β promoter was evaluated with the Dual-Luciferase assay. Three independent experiments were performed with similar results. Error bars show standard deviations. *, P < 0.05; **, P < 0.01.