FIG 9.

E120R impaired the interaction between IRF3 and TBK1. (A) PK-15 cells were transfected with 10 μg of FLAG vector or 5 μg or 10 μg of FLAG-E120R or 10 μg of FLAG-E120R dm expression plasmid. At 24 hpt, cells were transfected with poly(dA·dT) (2 μg/ml) for 12 h. Cells were lysed, and the lysates were immunoprecipitated with anti-IRF3 antibody and subjected to Western blotting. The antibody-antigen complexes were detected using anti-TBK1, anti-IRF3, or anti-FLAG antibody. Three independent experiments were performed with similar results. (B) PAMs were mock infected or infected with ASFV WT or ASFV E120R-Δ72-73aa (MOI of 1) for 8 h. Cell lysates were immunoprecipitated with anti-IRF3 antibody and subjected to Western blotting. The antibody-antigen complexes were detected using anti-TBK1, anti-IRF3, or anti-E120R antibody. Three independent experiments were performed with similar results. IP, immunoprecipitation; IB, immunoblotting.