FIG 3.

Porcine epidemic diarrhea virus infection upregulated histone acetylation by inhibition of HDAC1 expression. (A) Far-Western detection of total acetylated proteins in IPEC-J2 cells infected with PEDV (MOI of 0.1 and 1) using an acetylated lysine antibody. Histone H3 and β-actin were used as loading controls. (B) Acetylation of specific lysine residues of histone H3 (H3K27 and H3K9) and expression of PEDV N and HDAC1 in PEDV-infected cells shown by Western blotting. Mock cells were used as the control. (C) H3K27ac and HDAC1 expression in IPEC-J2 cells treated with HDAC1 inhibitor MS-275 at different concentrations. (D) H3K27 acetylation level in cells infected with PEDV or treated with MS-275. (E) Ratio of H3K27ac to histone H3 protein of three independent experiments as shown in panel C. (F) H3K27 acetylation level and HDAC1 expression in the cells infected with PEDV or those expressing exogenous HDAC1 with or without MS-275 treatment. Histone H3 and β-actin were used as loading controls. Data were shown as means ± SD from three independent experiments for panel D. *, P < 0.05; **, P < 0.01.