FIG 7.

Identification of proteins of porcine epidemic diarrhea virus that downregulate HDAC1 transcription. (A) Heat map showing the effect of different PEDV proteins on HDAC1 transcription in the IPEC-J2 cells transfected with recombinant pCMV-Flag plasmids containing coding sequences for different PEDV proteins. The vector without insert was used as a control. The experiment was repeated 3 times. (B) Effects of PEDV N overexpression on HADC1 expression and H3K27 acetylation as detected by Western blotting. Histone H3 and β-actin were used as loading controls. (C) Relative expression of HDAC1 to β-actin by densitometric analysis shown as means ± SD from three independent experiments. **, P < 0.01.