FIG. 2.

Analysis of the Fli-1 transcription initiation site(s) by 5′ RACE (A) and primer extension (B) techniques, using primer NFli3′. (A) Electrophoretic analysis of 5′ RACE reaction products (see Materials and Methods) obtained by using poly(A)⁺ mRNA prepared from CB7 or 745-A cells. Arrowheads indicate the main reaction products. Sizes of molecular weight markers are shown in base pairs on the left. (B) Electrophoretic analysis of extension products obtained from total RNA prepared from the indicated cell lines. The relative (to the ATG codon) positions of main extension products in F-MuLV- and SFFV-transformed lines are shown on the right. Sizes of molecular weight markers (pUC19/HpaII) are shown in base pairs on the left.