Map of Fli-1 transcription initiation sites determined by 5′ RACE, S1 nuclease protection, or RNase protection assays in F-MuLV-transformed, SFFV-transformed lines, or normal mouse spleen cells. The 5′ region of the Fli-1 gene is schematically shown at the top. The relative abundance of the transcripts initiated at these different sites in tested cells is illustrated by the thickness of the vertical arrows. Ex1, exon 1 coding sequence.