Figure 1.

Cre-lox recombination results in ChR2 expression in the majority of CRH-expressing neurons in the PMC. A, Schematic of coronal section through mouse brain at the level of the pons illustrating the location of the pontine micturition center (green) and the adjacent locus ceruleus (red). B, Using RNAscope to perform ISH, probes targeting the mRNA for CRH identify PMC (green) and probes targeting the mRNA for TH identifies the LC (red). C, In situ hybridization (ISH) of CRH mRNA (red) and ChR2 (green) shows significant overlap of neurons with both colors in PMC (200×). Scale bar, 50 µm. First panel shows DAPI (blue) only, second panel CRH (red) only, third panel ChR2 (green) only, and fourth panel overlap of all 3 channels.