Figure 3.

Localization of the iron exporter Fpn in normal axons. Teased nerve fiber preparations of uninjured sciatic nerve from adult WT mice stained for (i) Fpn (green) and (ii) rhodamine-conjugated phalloidin (red), which strongly labeled F-actin at Schmidt-Lanterman incisures and nodes of Ranvier. The node of Ranvier (arrow) and the Schmidt-Lanterman incisure (arrowhead) can be distinguished by their characteristic morphology (constricted ring and funnel shape, respectively). Staining for Fpn was mainly localized to these two sites (iii; merged image), being slightly more intense at paranodal loop regions of the nodes of Ranvier than at the Schmidt-Lanterman incisures. Scale bar, 15 µm.