Figure 4.

Localization of the iron importer TfR1 in normal axons. Teased nerve fiber preparations of uninjured sciatic nerve from adult WT mice stained for TfR1 (green) and rhodamine-conjugated phalloidin (red) to label F-actin localized at nodes of Ranvier (arrow) and Schmidt-Lanterman incisures (arrowhead). TfR1 staining (i) was mainly localized along the axon (not the surrounding Schwann cell or myelin), with increased staining at the region near the node of Ranvier (arrows) (ii,iii). In some preparations, in which the Schwann cell bodies were visible (iv-vi), TfR1 staining was prominent at the cell body (arrows) as detected by the presence of DAPI-labeled Schwann cell nucleus (v) and the TfR1 labeling, which appeared to wrap around the axon (v,vi). Scale bars: iii, 15 µm; vi, 10 µm.