Figure 8.

Histologic identification of lentiviral expression and infected target cells using immunofluorescence staining with GFP and NeuN, GFAP, or Iba-1 in the CeA. Staining was performed on day 35 post-SNL/sham SNL surgery. Shown are representative images for the immunofluorescence staining of GFP (green) with NeuN (a neuronal marker), GFAP (an astrocyte marker), Iba-1 (a microglial marker), and DAPI (the nuclear marker) in the CeA tissue in sham-operated or SNL-operated rats, which received microinjection of lentivirus-expressing pep2-EVKI, pep2-SVKE, Tat-GluA2_(3Y), and Tat-GluA2_(3A), respectively. Scale bar, 25 μm. n = 3 biological replicates.