Supplementary Fig. 3.

Dose-dependent neutralization activity against SARS-CoV-2pp containing variant mutations N501Y/E484K/K417N. Neutralization activity of mouse sera were tested at 1:50 to 1:109,350 in 3 fold dilution against lentivirus particles pseudotyped with either spike of SARS-CoV-2(WT) or S encoding triple mutations N501Y/E484K/K417N. Pre- (pooled) or Post-vaccination sera were pre-incubated with pseudoparticles (PP) encoding different surface proteins for 1 hour followed by addition to 293 ACE-2 cells. 48 hour post-transduction, entry of PP were quantitated by luciferase activity. Result are done in duplicate normalized to entry of PP in presence of PBS. Representative of two independent experiments is shown.