Figure 2.

Schematic of print plate layout along with corresponding array layout using the provided print plan and print design

(A) Layout of the print plate. 20 antigens and their dilutions are placed in alternating positions as indicated by the different colors.

(B) Arrangement of the antigens on the microarray. Antigen from the print plate has the same color in the microarray arrangement. The 20 antigens included can be seen in different colors, each represented by three spots. The different sizes are representing different dilutions. Layout of the array is randomized to prevent spatial effects. If a new print plan and print design was produced by the provided shiny-based application, the layout would be altered correspondingly.