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. 2021 Aug 9;6(9):1175–1187. doi: 10.1038/s41564-021-00949-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Immunostaining of CcrZ in wild type S. pneumoniae shows a septal localization. Scale bar, 3 µm. b, Localization of CcrZ in other pneumococcal strains (un-encapsulated R6 strain and capsular serotype 4 TIGR4) and in S. aureus SH1000, as well as in B. subtilis 1A700. Left and top-right scale bars, 3 µm; bottom-right scale bar, 5 µm. c, 3D-SIM of GFP-CcrZ (green) and FtsZ-mCherry (red) and reconstructed volume projection of both. Left scale bar, 2 µm; right scale bar, 500 nm.