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. 2021 Aug 11;596(7873):553–557. doi: 10.1038/s41586-021-03814-7

Fig. 2. Shepherding and nest entry activate virgin PVN/OT-PVN neurons.

Fig. 2

a, Left, DREADDi–mCherry in OT-PVN cells. Right, CNO treatment during initial co-housing reduced retrieval in DREADDi-expressing mice. b, Optically tagged OT-PVN neurons in vivo. Left, µCT image used to localize electrodes. Middle, ChETA–eGFP expression in OT-PVN neurons. Right, raster plot of photo-tagged OT-PVN neuron; inset, waveform; blue bar, light pulse. c, d, Raster plot (c) and before–after plot (d) of PVN single unit from co-housed virgin during shepherding (shading). e, Change in spiking rate during shepherding for individual units. Filled bars indicate significant changes in spiking rate. f, Units with significant rate changes during shepherding (red) fired less during virgin→dam chases (grey). g, Simultaneously recorded PVN cells including OT-PVN cell (u3) during nest entry event. Units are numbered u1–u9. h, u3 spiking during nest entry (n = 140 events). i, Firing rate during nest entry. j, Spiking rate when virgin entered nest after shepherding (red) or voluntarily (grey). Data are mean ± s.e.m.; *P < 0.05, **P < 0.01.