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. 2021 Aug 11;596(7873):553–557. doi: 10.1038/s41586-021-03814-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Recordings in female mouse PVN brain slices. Left, current-clamp whole-cell recording from OT-PVN neuron expressing DREADDi-mCherry before (top) and after (bottom) CNO bath administration. Right, summary of chemogenetic suppression of OT-PVN neuronal firing in brain slices (‘hM4D(Di)-mCherry’ baseline firing rate before CNO: 8.8±1.7 Hz, after CNO: 0.6±0.1 Hz, n=7 cells from N=4 mice; ‘mCherry’ baseline firing rate before CNO: 4.8±0.6 Hz, after CNO: 5.0±0.9 Hz, n=8 cells from N=3 mice; two-way ANOVA with Sidak’s correction for multiple comparisons, p=0.0002 between baseline and CNO for hM4D(Di)-mCherry, p=0.9 between baseline and CNO for mCherry). Error bars, SEM. b, CNO in retrieving females that expressed DREADDi did not affect retrieval rates (baseline retrieval: 76.0±9.2%, N=5; CNO: 72.0±18.8%, N=5; saline: 92.0±8.0%, N=5; repeated measures ANOVA, p=0.332). Error bars, SEM.