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. 2021 Jul 15;6(9):1129–1139. doi: 10.1038/s41564-021-00934-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Slice through a tomogram of a K. stuttgartiensis cell. Elongated structures such as those inside the blue box are anammoxosomal tubules; the fourfold symmetrical particles are hydrazine dehydrogenase (HDH) complexes⁷⁶. Scale bar, 100 nm. Shown is a slice from one representative tomogram of 19. b, A 22 Å resolution subtomogram average of anammoxosomal tubules. A side and top view of the tubule are shown. c, Fit of KsNXR-ABC trimers (cartoon) into the subtomogram averaging map. d, The connecting density (circled) is not explained by KsNXR-ABC. e,f, Negative-stain electron micrographs of KsNXR-ABC alone (e) and after incubation with KsNXR-T (f). Scale bars, 100 nm. Shown are representative micrographs out of three independent reconstitution reactions.