Extended Data Fig. 5. Proteomics of anammoxosomal tubules.
a, Negative stain electron micrograph of anammoxosomal tubules purified from K. stuttgartiensis. b, as panel a but after incubation with DDM. (scale bars: 100 nm). Shown are representative micrographs from three independent experiments. c, Coomassie-stained SDS-PAGE gel of the tubule preparation. The left lane (M) shows molecular weight markers, with their apparent MW indicated in kDa. The middle and right lanes show the tubule preparation before and after density gradient centrifugation, with the proteins labeled as identified by peptide mass fingerprinting. The outer membrane porin Kustd1878 is no longer present after the centrifugation step, whereas NXR-T is. Tubule purification and analysis was performed twice, showing the presence of NXR-T each time. d, nitrite oxidation assay results on purified anammoxosomal tubules incubated at 35 °C without (-DDM) and with (+DDM) the detergent dodecyl maltoside. Assays were performed in triplicates; the bar graps shows the average, the error bar represents the standard deviation and individual datapoints are shown as asterisks.