TABLE 3.

Kinds and locations of unequivocally independent point mutations induced in the lacZα gene during replication of UV-irradiated M13mp2-oriL by XP variant cell extracts^a

Mutant	Position(s)b	Mutation	Photoproduct context (5′→3′)d	Strandc
XU65	84	C · G→A · T	CAGTCACGA	Leading
XU49	90	C · G→A · T	TTTTCCCAG	Leading
XU101	90	C · G→A · T	TTTTCCCAG	Leading
XU41	90	C · G→A · T	TTTTCCCAG	Leading
XU81	145	C · G→A · T	CCAGCTGGC	Leading
XU84	145	C · G→A · T	CCAGCTGGC	Leading
XU97	148	C · G→A · T	ACGCCAGCT	Leading
XU47	159	C · G→A · T	TCTTCGCTA	Leading
XU59	162	C · G→A · T	GCCTCTTCG	Leading
XU105	88	C · G→T · A	TTCCCAGTC	Leading
XU107	107–108	C · G→T · A, C · G→T · A	TTACCCAAC	Lagging
XU102	108	C · G→T · A	TACCCAACT	Lagging
XU91	108	C · G→T · A	TACCCAACT	Lagging
XU66	108	C · G→T · A	TACCCAACT	Lagging
XU7	32	T · A→A · T	CTGTTTCCT	Leading
XU73	54	T · A→A · T	GAATTCGTA	Leading
XU106	56	T · A→A · T	CGAATTCAC	Lagging
XU67	67	T · A→A · T	GCCGTCGTT	Lagging
XU74	73	T · A→A · T	GTTTTACAA	Lagging
XU29	54	T · A→C · G	GAATTCGTA	Leading
XU55	74–75	ΔAC	TTTTACAAC	Lagging
XU69	132–136	ΔC	CATCCCCCT	Lagging

^aThe target genes from 45 mutants were sequenced by standard methods. Of these, 10 had no mutation in the target gene and 2 had large deletions. Twelve mutants had mutations that were also found in unreplicated templates, i.e., that represented background mutations. These 12 were omitted from the analysis and from calculations of UV-induced mutant frequency. 

^bThe lacZα gene comprises positions −84 to +174, with +40 being the first transcribed nucleotide. 

^c“Strand” refers to the newly synthesized DNA that contains a mutation as a result of error-prone bypass of photoproducts that were located in the corresponding template strand. 

^dThe site of the mutation is underlined.