TABLE 4.

Kinds and locations of unequivocally independent point mutations induced in the lacZα gene during replication of UV-irradiated M13mp2-oriL by extracts from HeLa cells^a or MSU-1.2 cells^b

Mutant	Position(s)	Mutation	Photoproduct context (5′→3′)d	Strandc
HeLa
HU63	−57	C · G→T · A	AGCTCACTC	Lagging
HU64	−57	C · G→T · A	AGCTCACTC	Lagging
HU49	−57	C · G→T · A	AGCTCACTC	Lagging
HU59	−57	C · G→T · A	AGCTCACTC	Lagging
HU36	29	C · G→T · A	TTTCCTGTG	Leading
HU34	88	C · G→T · A	TTTCCCAGT	Leading
HU39	88	C · G→T · A	TTTCCCAGT	Leading
HU70	89	C · G→T · A	TTTCCCAGT	Leading
HU67	90	C · G→T · A	TTTCCCAGT	Leading
HU51	90	C · G→T · A	TTTCCCAGT	Leading
HU43	96	C · G→T · A	AAACCCTGG	Lagging
HU69	108	C · G→T · A	TACCCAACT	Lagging
HU33	108	C · G→T · A	TACCCAACT	Lagging
HU42	108	C · G→T · A	TACCCAACT	Lagging
HU53	145	C · G→T · A	CCAGCTGGC	Leading
HU60	149	C · G→T · A	TACGCCAGC	Leading
HU47	30	C · G→G · C	GTTTCCTGT	Leading
HU37	89	C · G→G · C	TTTCCCAGT	Leading
HU57	159	C · G→A · T	TCTTCGCTA	Leading
HU68	42	T · A→C · G	ATGGTCATA	Leading
HU55	91	T · A→C · G	GTTTTCCCA	Leading
HU40	106–108	ΔC	TTACCCAAC	Lagging
MSU-1.2
NU7	−57	C · G→T · A	AGCTCACTC	Lagging
NU34	75	C · G→T · A	TTTACACG	Lagging
NU1	89	G · G→T · A	TTTCCCAGT	Leading
NU3	89	C · G→T · A	TTTCCCAGT	Leading
NU18	89	C · G→T · A	TTTCCCAGT	Leading
NU11	90	C · G→T · A	TTTCCCAGT	Leading
NU35	108	C · G→T · A	TACCCAACT	Lagging
NU21	108	C · G→T · A	TACCCAACT	Lagging
NU2	145	C · G→T · A	CCAGCTGGC	Leading
NU22	−57	C · G→A · T	AGCTCACTC	Lagging
NU31	85	T · A→C · G	CCAGTCAC	Leading
NU17	131	T · A→C · G	CGACATCCC	Lagging
NU34	62–63	ΔC	CGGCCAGTC	Leading

^aThe target genes from 44 mutants derived with HeLa cell extracts were sequenced by standard methods. Of these, eight had no mutation in the target gene and two had large deletions. Twelve background mutations were omitted from the analysis. 

^bThe target genes from 23 mutants derived with MSU-1.2 cell extracts were sequenced. Of these, six had no mutation in the target gene, three had background mutations, which were omitted from the analysis, and one had an 11-bp insertion between positions 57 and 58. 

^cStrand, as in Table 3. 

^dThe site of the mutation is underlined.