TABLE 6.

Kinds and locations of unequivocally independent point mutations induced in the lacZα gene during replication of UV-irradiated M13mp2-oriR by HeLa cell extracts^a

Mutant	Position(s)	Mutation	Photoproduct context (5′→3′)c	Strandb
HR14	−57	C · G→T · A	AGCTCACTC	Leading
HR25	58	C · G→T · A	AATTCACTG	Leading
HR15	58	C · G→T · A	AATTCACTG	Leading
HR5	75	C · G→T · A	TTTACAACG	Leading
HR9	88	C · G→T · A	TTTCCCAGT	Lagging
HR11	88	C · G→T · A	TTTCCCAGT	Lagging
HR19	89	C · G→T · A	TTTCCCAGT	Lagging
HR6	89	C · G→T · A	TTTCCCAGT	Lagging
HR20	90	C · G→T · A	TTTCCCAGT	Lagging
HR7	90	C · G→T · A	TTTCCCAGT	Lagging
HR10	90	C · G→T · A	TTTCCCAGT	Lagging
HR13	90	C · G→T · A	TTTCCCAGT	Lagging
HR8	96	C · G→T · A	AAACCCTGG	Leading
HR12	96	C · G→T · A	AAACCCTGG	Leading
HR24	107–108	C · G→T · A, C · G→T · A	TTACCCAAC	Leading
HR16	108	C · G→T · A	TACCCAACT	Leading
HR26	120	C · G→T · A	TCGCCTTGC	Leading
HR23	129	C · G→T · A	AGCACATCC	Leading
HR27	84	C · G→A · T	CCAGTCACG	Lagging
HR22	115	T · A→A · T	GCGATTAAG	Lagging
HR17	132–136	ΔC	CATCCCCCT	Leading

^aThe target genes from 37 mutants derived with HeLa cell extracts were sequenced. Of these, 10 had no mutation in the target gene and one had a large deletion. Three had background mutations and were omitted from the analysis. 

^bStrand, as in Table 3. 

^cThe site of the mutation is underlined.