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. 2021 Aug 25;4:1007. doi: 10.1038/s42003-021-02541-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Location of crRNAs target sites in the phaA and bktB genes. They are co-transcribed from the A of the start codon ATG. t1 targets the template strand of phaA in promoter region. c1 and c2 targets the coding strand of bktB. b Changes in the transcriptional levels of phaA and bktB in DF50ΔEPSΔcas3 individually expressing phaA-t1, bktB-c1and, bktB-c1 or simultaneously expressing phaA-t1and bktB-c2. Data shown for three biological replicates. Error bars indicate SDs, n = 3.