Fig. 6. Continuous inhibition of citZ and gltA and the cell growth, glucose consumption of strains with these two genes repressed.
a The repression of citZ by citZ-t2 and gltA by gltA-t1 during the strain cultivation. b Changes in the transcriptional levels of citZ and gltA in DF50ΔEPSΔcas3 simultaneously expressing citZ-t2 and gltA-t1. c The cell growth of DF50ΔEPSΔcas3 with citrate synthase genes repressed. d Glucose consumption of DF50ΔEPSΔcas3 with citrate synthase genes repressed. CS represents DF50ΔEPSΔcas3 expressing both citZ-t2 and gltA-t1. Data shown for three biological replicates. Error bars indicate SDs, n = 3.