(A) Representative flow cytometry plots of the expression of CCR6, CCR4, CXCR3 and CCR10 on total CD4+ T cells in one individual. The overlays indicate the antigen specific CD4+ T cells producing IFN-γ (blue), IL-22 (red) or IL-17A (green). The frequencies of each subset are indicated. (B) The chemokine receptor distribution of cells producing IFN-γ (blue), IL-22 (red) or IL-17A (green) in response to M.tb lysate (n=19, 19 and 11, respectively). Only individuals with a positive cytokine response and more than 30 cytokine events were included in the phenotyping. Each dot represents one individual. Data are shown as box and whisker (interquartile range) plots and horizontal bars represent the median. Statistical comparisons were performed using Kruskal-Wallis and Dunn’s multiple comparison test. *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001