Figure 5.
SIRPα modulates multiple downstream signaling pathways during M1 macrophage polarization. A) The phosphorylation of P65, IKKα/β, ERK1/2, P38, JNK, STAT1, Akt1 and Akt2 in WT and Sirpα−/− BMDM in the absence or presence of mCD47.ex during M1 polarization. B) Signaling transduction in WT and Sirpα−/− BMDM in the absence or presence of mCD47.ex after LPS, CpG or Poly(I:C) treatment. C) The co-association of p85, SHP-1, and SIRPα during M1 activation. D) Model of SIRPα activation and its modulation on PI3K-Akt signaling through SHP-1 under M1 macrophage polarization.