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. Author manuscript; available in PMC: 2022 Feb 2.
Published in final edited form as: Nat Chem Biol. 2021 Aug 2;17(9):998–1007. doi: 10.1038/s41589-021-00840-4

Figure 3. Control of cell proliferation by induced target sequestration.

Figure 3.

a, Scheme for rapamycin inducible client recruitment: Cdc24-mScarlet-FKBP to FRB-(RGG)3 scaffold condensates. b, Representative images of cells expressing Cdc24-mScarlet-FKBP and FRB-Scaffold before (top) and after (bottom) rapamycin addition. c, Quantification of fraction of scaffold and client (Cdc24) in synthetic condensates over time as in b, for 16 cells. Shaded area, 95% CI. d, Spot dilution assays of the same yeast strain grown, containing Cdc24-mScarlet-FKBP, either lacking or expressing condensates, without or with 100 nM rapamycin in the media.