Skip to main content
. Author manuscript; available in PMC: 2022 Feb 2.
Published in final edited form as: Nat Chem Biol. 2021 Aug 2;17(9):998–1007. doi: 10.1038/s41589-021-00840-4

Extended Data Fig. 3. Reversible control of cell proliferation-arrest state.

Extended Data Fig. 3

a, Representative images of Cdc24-mScarlet-TsCC(B) cells expressing TsCC(A)-(RGG)3 scaffold at the indicated temperatures for 14 hours. Thermally responsive coiled-coil pair dissociate upon heating to 42°C, releasing client to promote cell polarity and reversing the cell cycle arrest associated with Cdc24 sequestration to condensates. b, Representative images of Cdc24-mScarlet-TsCC(B) in the presence of TsCC(A)-PhoCl2f-(RGG)3 before and after illumination with 405 nm light. c, Schematic of client release strategy: Cdc24 is tagged with PhoCl-TsCC(B). 405 nm light results in PhoCl cleavage and client release. d, Percentage of cells expressing Cdc24-mScarlet-TsCC(B) arrested (unbudded cells) over time after scaffold induction +/− illumination. n = 4048 cells in total pooled from three trials. e, Prediction: cycling of cell state between budded-arrested-budded-arrested. f, Representative images of cells at the indicated time points. Wildtype levels of budding at time 0 h. Cells incubated in galactose at 25°C from 0–6h timepoints to induce condensate formation, blocking budding, then heated from 6h to 8h timepoints, promoting polarization and budding and cooled back to 25°C and arrested by 12h.