Extended Data Fig. 4. Sequestration to synthetic condensates in mammalian cells.

a, Schematic of CRISPR tagging approach to endogenous loci in mammalian cells and expression of the scaffold by a CMV promoter. b, PCR validation of CRISPR tagging in mammalian cell lines. Only tagged strains show a PCR product of the expected size as indicated. c, Representative images of tagged ERK1 robustly partitions to synthetic condensates formed by expression of TsCC(A)-(RGG)₃ scaffold. d, Representative images of tagged Par6 localized to the cell cortex in the absence of scaffold expression (left) and to condensate structures when scaffold is expressed (right). e, Quantitation of cortical Par6-mCherry-TsCC(B) in the absence and presence of condensates with cognate coiled coil. n = 10 cells for each condition. Error bars, s.d. Significance calculated by unpaired, two-tailed t-test. (**, p ≤ 0.01).