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(A) Mold design with CAD software; (B) Mold printing; (C) PDMS casting on the mold; (D) Assembled bioreactor; (E) Cross-section view of organoid loading procedure whereby microchannels were filled with media first, then an organoid was placed in the open well, and the wells were sealed using adhesive optical film; (F) On-chip culturing system assembly.
