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. 2021 Aug 25;12:5110. doi: 10.1038/s41467-021-25404-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Binding analysis of TCRs A3.10, A1.9, A2.13, and A5.5 for a HLA-DQ8-CpepL11C and b HLA-DQ8-HIPL11C was determined using SPR. A concentration series from 200 μM to 0 μM of each TCR was passed over surface immobilised a HLA-DQ8-CpepL11C and b HLA-DQ8-HIPL11C. Left columns in a and b: measured response curves of the single dilution series for each TCR. Right columns in a and b: Curve fits for TCR-HLA-DQ8-CpepL11C/HIPL11C and K_D determination using single ligand binding model. Each TCR sample was analysed in duplicate, and three (n = 3) or four (n = 4) (as indicated) independent experiments were conducted and measurements were combined after normalising each equilibrium response curve against the calculated response maximum. Data are mean ± standard error, s.e.m. Source data are provided as a Source Data file.