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. 2021 Jul 7;108(8):1466–1477. doi: 10.1016/j.ajhg.2021.06.010

Figure 4.

Figure 4

Sperm ultrastructure analyses for men harboring bi-allelic DNAH10 variants

TEM analysis of spermatozoa obtained from a fertile control individual (NC) and men harboring bi-allelic DNAH10 variants. Cross-sections of the midpiece and principal piece of the sperm flagella in the sperm obtained from NC displayed typical “9 + 2” microtubule structure and peri-axoneme structure. The axoneme microtubule structure, including nine pairs of peripheral doublet microtubules (DMT; indicated with white arrows) and the central pair of microtubules (CP; indicated with blue arrows), is visible. The outer dynein arms (ODA; indicated with yellow arrows) and inner dynein arms (IDA; indicated with red arrows) are also visible. The peri-axoneme structure includes a helical mitochondrial sheath (MS; indicated with green arrows), nine outer dense fibers (ODFs; indicated with orange arrows), and the fiber sheath (FS; indicated with pink arrows). Cross-sections of the midpiece, principal piece, and endpiece of the spermatozoa obtained from men harboring bi-allelic DNAH10 variants revealed typical axonemal anomalies with the absence of inner dynein arms, while other axoneme microtubule structures seemed to be unaffected. The data of NK067 II-1 and NK067 II-2 are shown as an example. Scale bars, 200 nm.